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The WAVE System Model 4500 is one of the most sensitive and accurate
technologies for separation and sequence variant analysis of nucleic acid
fragments. As a proven technology, the WAVE System is used by many of the
world.s leading medical centers. The WAVE 4500 System is fully automated, from
sample collection to results analysis. Navigator. Software is included and
creates the fragment specific methods, controls the instrument and analyzes
your results. Fragment separation is achieved by the patented DNASep® Cartridge
located in the system oven. The fragments pass through the system’s ultraviolet
detector and absorbance is recorded over time. This information is processed by
the Navigator Software suite. It records the results in the form of
chromatograms that can be further analyzed and filed by Navigator. Screening by
WAVE system reduces the sequencing costs and increases the speed of variant
analysis in a large samples or gene with many exons, eg BRCA1 & 2, MSH1 & MLH2.
The system can process 2 x 96 well sample plate unattended for Mutational
Analysis and the average run time is < 5 mins for each sample
The Fragment collector FCW 200 connected to the WAVE 4500 – can collect the
separated fragment, by clicking the peak of your interest for further processing
such as sequence analysis.
Modes of Operation
The WAVE System can operate under three modes based on the temperature in the
oven, allowing multiple applications with one system. Navigator Software
provides a platform for simple method development for each application as well
as substantial flexibility for novel procedures
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- Detect low frequency of variants in the heterogeneous samples
- Detect tumor-associated somatic mutations despite presence of
normal cells/tissues
- Pool samples to increase efficiency of screening for SNPs
- Study mitochondrial heteroplasmy by finding mutations at less
than 1% abundance
- Pool samples to increase efficiency of screening for SNPs
- Study mitochondrial heteroplasmy by finding mutations at less
than 1% abundance
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Applications of WAVE 4500 DHPLC System
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Condition |
Temperature |
Separation Type |
Applications |
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Non-denaturing |
40o C - 50o C
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Size-dependent |
Size-dependent sizing of double-stranded DNA
PCR quality check
STR, RFLP, AFLP sizing Quantitative analysis (Q-RT-PCR)
Separation & prrification of DNA fragments |
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Partially Denaturing |
52o C - 75o C |
Sequence-dependent |
Variation Discovery
Variation Detection
Methylation Detection
Purification of heteroduplex DNA for sequencing |
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Fully Denaturing |
70o C - 100o C |
Size-dependent_Sequence-dependent |
Separation of single-stranded nucleic acids
Single-base
extension/mini-sequencing
Oligonucleotide analysis/purification |
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Description |
Quantity |
Size |
Catalog Number |
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WAVE Optimized TEAA Buffer A-Case |
4 x 2.5 liter bottles |
1000 injections |
553401 |
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WAVE Optimized TEAA Buffer B - Case |
4 x 2.5 liter bottles |
1000 injections |
553402 |
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WAVE Optimized Solution D - Case |
4 x 2.5 liter bottles |
1000 injections |
553412 |
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DNASep® Cartridge |
4.6 mm x 50 mm |
4000 injections |
DNA-99-3510 |
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WAVE®DNA Sizing Standard Sizing |
Sizing Standard for WAVE Systems (pUC18 HaeIII Digest) |
180 μL, 20 testings |
560078 |
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WAVE® Low Range Mutation Standard, 56o C |
209 bp Mutation Standard DYS 271 |
180 μL, 20 testings |
560077 |
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SURVEYOR Mutation Detection Kit |
30μL |
25 reactions |
706035 |
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MitoScreen Assay Kit |
55μL |
12 complete mitochondial genome scans |
707001 |
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Optimase Polymerase Supplied with buffer containing Mg++ |
100μL |
1 x 250 Units |
703030 |
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MicroAmp 96well PCR Plate |
10 x 96 well plates |
10 plates/pack |
N801-0560 |
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