Applications:

• DNA Sequencing
• Fragment Analysis (Genotyping)
• Mutation/Heterozgote Identification.
• SNP validation
• Microbial Identification
• AFLP / RFLP Analysis
• HIV-1 Drug Resistant Genotyping
• Microsatellite Analysis
• Forensic Identification
• SAGE (Serial Analysis of Gene Expression)

The ABI 3130 Genetic Analyzer will be used for high class molecular biology research work to meet the DNA Sequencing and Fragment Analysis needs for various applications in the field of Human Genetics, Cancer Research, Microbial Identification and Human Identification

Overview of the Genetic Analyzer :

This laser based 4 capillary Genetic Analyzer can sequence 96 samples / day without any operator intervention, The Automated Polymer Delivery System eliminates manual washing and filling of polymer syringes, significantly reducing the time required for instrument setup and maintenance. All steps are automated, including polymer loading, sample injection, separation and detection, and data analysis.

Both DNA Sequencing and Fragment Analysis can be performed on the POP7 polymer using a 50 cm capillary array. Data is collected by a high resolution CCD camera during the run and analyzed. The analyzed files are then available for direct transfer to a database for further post analysis using specific software’s for identification and reporting.

Genetic Analyzer Software :

1. Data Collection v 3.0 : Interface between the Genetic Analyzer and the Data station, which controls the Genetic Analyzer. The run modules and plate setup is performed using this Data collection software and to monitor the run progress.
2. Sequencing Analysis Software v 5.2—designed to analyze, display, edit, save, and print sequencing data. Generation of quality values provides accurate basecall information for pure and mixed base calls.
3. SeqScape® Software v.2.5—used for mutation detection and profiling. Basecalling algorithms seek out mixed bases for comparative sequencing, SNP discovery, and validation. With comparisons to a reference sequence, many samples can be analyzed and assembled simultaneously for fast and accurate determination of mutations.
4. GeneMapper® Software v.4.0 —the most comprehensive fragment analysis software available. Multiple application features make it an ideal tool for genotyping, allele calling, fragment sizing, and SNP analysis. Quality Values (QV) are assigned to each fragment analyzed for easy automation and throughput, and questionable fragments are easily identified. Applications include Microsatellite analysis (diploid and polyploid), linkage mapping, SNP analysis (including SNaPshot® Kits), AFLP, relative fluorescent quantitation including loss of heterozygosity, and conformational sizing.

List of Reagents for ABI 3130 Genetic Analzyer

Applications

De-novo Sequencing

Sequencing of PCR Products ‎

Mitochondrial DNA Sequencing Products

This PCR based resequencing system identifies human mitochondrial genetic variations.

VariantSEQr ReSequencing System

Primer sets formulated at working concentrations for hundreds of disease-related human genetic variants. Reference sequences for all amplicons provided for auto analysis.

Microbial Identification

The MicroSeq® Microbial Identification System is a complete solution for all eubacteria, yeasts, and filamentous fungi. It rapidly identifies slow-growers and non-fermenters (in less than 24 hours) that are often impossible to identify with conventional systems. No prior knowledge of the organism is required since the system uses universal, domain-level primers.

Fragment Analysis

Microsatellite Analysis (LOH & MIN)

Microsatellites, also known as short tandem repeats (STRs), are polymorphic DNA loci that contain a repeat sequence of 2–7 nucleotides. The number of repeats for a given locus may vary, resulting in alleles of differing lengths.

SNaPshot Multiplex System

SNaPshot Multiplex System is a primer extension based method that enables multiplexing to 10 SNPs. Interrogate multiple SNPs regardless of chromosome position. Could be used ‎to screen and confirm SNP, assess DNA methylation, finger print BACs.

Human Linkage Mapping System

ABI Prism Linkage Mapping set v2.5 consists of fluorescently labeled PCR primers selected to amplify highly informative di-repeat Microsatellite loci from Genethon human linkage map.

Human Identification (Forensic application)

Fluorescent STR-based assay for human identification applications. The AmpFlSTR Identifiler PCR Amplification Kit simultaneously amplifies 15 STR loci plus the Amelogenin gender-determining marker in a single, robust PCR. The widely accepted ‎tetranucleotide loci co-amplified in the Identifiler Kit include the thirteen core STR loci as ‎required for sample entry into CODIS (Combined DNA Index System):