Overview

The Applied Biosystems 7500 Fast Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. Sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

Applications

• Absolute quantitation method determines target quantity directly from a standard curve. To quantitate the amount of target in unknown samples, the system measures the sample’s CT and uses a standard curve to determine starting copy number.
• The Relative quantitation eliminates the need for a standard curve by calculating quantitation values of an unknown sample relative to a calibration sample. Relative quantitation is ideal for gene expression studies. An endogenous control is used to normalize the sample amount. The relative quantitation (RQ) study, which comes standard, allows simultaneous analysis of up to ten plates of data.
• Screening of known SNPs, complete system for SNP genotyping and gene expression studies.
• Plus/minus assay.