Genomics Automated Fluorescent &Genetic Analyzer

Model : ABI 3130 Genetic Analyzer Make : M/s. Applied Biosystems, USA Location : Genomics Lab.

Applications:

• DNA Sequencing
• Fragment Analysis (Genotyping)
• Mutation/Heterozgote Identification.
• SNP validation
• Microbial Identification
• AFLP / RFLP Analysis
• HIV-1 Drug Resistant Genotyping
• Microsatellite Analysis
• Forensic Identification
• SAGE (Serial Analysis of Gene Expression)

The ABI 3130 Genetic Analyzer will be used for high class molecular biology research work to meet the DNA Sequencing and Fragment Analysis needs for various applications in the field of Human Genetics, Cancer Research, Microbial Identification and Human Identification

Overview of the Genetic Analyzer :

This laser based 4 capillary Genetic Analyzer can sequence 96 samples / day without any operator intervention, The Automated Polymer Delivery System eliminates manual washing and filling of polymer syringes, significantly reducing the time required for instrument setup and maintenance. All steps are automated, including polymer loading, sample injection, separation and detection, and data analysis.

Both DNA Sequencing and Fragment Analysis can be performed on the POP7 polymer using a 50 cm capillary array. Data is collected by a high resolution CCD camera during the run and analyzed. The analyzed files are then available for direct transfer to a database for further post analysis using specific software’s for identification and reporting.

Genetic Analyzer Software :

1. Data Collection v 3.0 : Interface between the Genetic Analyzer and the Data station, which controls the Genetic Analyzer. The run modules and plate setup is performed using this Data collection software and to monitor the run progress.
2. Sequencing Analysis Software v 5.2—designed to analyze, display, edit, save, and print sequencing data. Generation of quality values provides accurate basecall information for pure and mixed base calls.
3. SeqScape® Software v.2.5—used for mutation detection and profiling. Basecalling algorithms seek out mixed bases for comparative sequencing, SNP discovery, and validation. With comparisons to a reference sequence, many samples can be analyzed and assembled simultaneously for fast and accurate determination of mutations.
4. GeneMapper® Software v.4.0 —the most comprehensive fragment analysis software available. Multiple application features make it an ideal tool for genotyping, allele calling, fragment sizing, and SNP analysis. Quality Values (QV) are assigned to each fragment analyzed for easy automation and throughput, and questionable fragments are easily identified. Applications include Microsatellite analysis (diploid and polyploid), linkage mapping, SNP analysis (including SNaPshot® Kits), AFLP, relative fluorescent quantitation including loss of heterozygosity, and conformational sizing.

List of Reagents for ABI 3130 Genetic Analzyer

Applications

De-novo Sequencing